The effects of osmotic agents on the morphology and integrity of the cell membrane Essay

Introduction

Cell membrane is the lone cellular construction which is found in every individual being on Earth. Membrane is what separate and maintains the life belongingss of the cell. It defines the cell.

The chief intent of this lab is to larn about membranes and understand how they function. Such as understanding the semi-permeability of cell membranes. The term semi-permeability means a membrane that is selectively permeable and allows merely certain molecules/ions to go through through. Furthermore, this lab besides enhances our cognition on the effects of osmosis on cells. Osmosis is a term that describes the self-generated motion of H2O across a semipermeable membrane from a part of lower concentration of solute to a part of a higher concentration of solute to do the solute concentration equal on both sides. Therefore, with the cognition of osmosis we can place whether solutions are hypoosmotic, hyperosmotic or isotonic by detecting the effects on cells. In this lab I predict… .

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Furthermore, this lab examines the effects of osmotic agents in the morphology of cell and observes membrane unity by exclusion of Trypan blue, a polar and bulky dye molecule.

Part A: attached with the hardcopy of the lab.

Part B: 1. Please refer to Postpone 2.1 in the hardcopy of the lab 2. Please refer to Postpone 2.1 in the hardcopy of the lab 3. I besides observed that the isosmotic solutions of glucose and K chloride are of different concentration for blood cells but they are of same concentration for works cells. The expected reply is that the isosmotic solutions of both glucose and potassium chloride should be of different concentrations due to difference in the Van’t Hoff factor ( I ) . Van’t Hoff factor is step of the figure of atoms released by a substance when dissolved. Since Potassium chloride releases 2 atoms ( K+ and Cl- ) when dissolved and glucose merely releases one atom when dissolved hence the expected isosmotic solutions of glucose and K chloride should be different. Based on the observation of Table2.1 my reply agrees to it when comparing isosmotic solutions for blood cells but it doesnot agree for works cells and that’s due to error in sensing through microscope.

Exercise 2.2

4. Based on the experimental informations and consequences from table 2.2, the individuality of my Unknown 1 is Glycine and the individuality of my unknown 2 is Methyl-?-cyclodextrin. The % bluish cells of unknown 1 is 38 % which is close to Glycine % blue cells which is 23 % . On the other manus unknown 2 is % bluish cells is 44.7 % which is near to % bluish cells of Methyl-?-cyclodextrin which is 78 % . Therefore, the individuality of my Unknown 1 is Glycine and the individuality of my unknown 2 is Methyl-?-cyclodextrin.

5. Based on the Trypan bluish exclusion check, Methyl-B-cyclodextrin is the compound which caused the most harm to the plasma membrane. Methyl-?-cyclodextrin is a compound that is used to badly deplete cells of cholesterin. Basically it removes cholesterin from membrane and disrupts membrane unity. Therefore, Methyl-?-cyclodextrin have disrupt the membrane unity of the cells and damage the plasma membrane. As a consequence, the trypan blue easy entered the cell and stained it.

DISCUSSION AND CONCLUSIONS ( 15 Markss entire ) Supply a treatment of your research lab observations and consequences. Include the undermentioned: 1 ) A brief and concise ( 100-150 word ) sum-up of your findings. Include the name of the checks that you performed, what you measured and the effects of any drugs or extra interventions that you performed. ( 5 Markss )

In the first portion of this lab, we identified whether the glucose and K chloride solutions are hypoosmotic, hyperosmotic or isotonic by detecting the effects on cell that whether the cells are shriveling or swelling. I besides observed that the isosmotic solutions of glucose and K chloride are of different concentration for blood cells but they are of same concentration for works cells. The expected reply is that the isosmotic solutions of both glucose and potassium chloride should be of different concentrations due to difference in the Van’t Hoff factor ( I ) . Based on the observation of Table2.1 my reply agrees to it when comparing isosmotic solutions for blood cells but it does non hold for works cells and that’s due to error in sensing through microscope.

In the 2nd portion of the experiment, we examines the effects of osmotic agents in the morphology of cell and observes membrane unity by exclusion of Trypan blue, a polar and bulky dye molecule. Based on table 2.2, % bluish cells of HPMI are 0 % , while % bluish cells of Glycine are 23 % , % bluish cells of Methyl-?-cyclodextrin are 78 % . By comparing my terra incognitas to the remainder, I observed that the individuality of my Unknown 1 is Glycine and the individuality of my unknown 2 is Methyl-?-cyclodextrin. The % bluish cells of unknown 1 is 38 % which is close to Glycine % blue cells which is 23 % . On the other manus unknown 2 is % bluish cells is 44.7 % which is near to % bluish cells of Methyl-?-cyclodextrin which is 78 % . Therefore, the individuality of my Unknown 1 is Glycine and the individuality of my unknown 2 is Methyl-?-cyclodextrin.

Therefore, this lab successfully identifies whether solutions are hypoosmotic, hyperosmotic or isotonic by detecting the effects on cells and successfully examines the effects of osmotic agents in the morphology of cell and observes membrane unity by exclusion of Trypan blue.

2 ) Supply an reply to the undermentioned inquiries:

  1. Mammalian cells are really sensitive to salt concentration. However, there are beings that thrive in really high-salt environments. What are they called and name one species at least? ( 2 Markss )

The beings that thrive in really high-salt environments are called Halophiles. The word Halophile itself describes its significance as Halophile comes from a Greek word which means salt-loving. ( https: //www.boundless.com/microbiology/textbooks/boundless-microbiology-textbook/microbial-evolution-phylogeny-and-diversity-8/euryarchaeota-111/extremely-halophilic-archaea-575-7678/ ) . Even though Halophiles are mostly classified into Archaea sphere, nevertheless there are alsohalophilic bacteriums and somehalophilic eucaryotes such as theDunaliella Salina, alga or the fungusWallemia ichthyophaga (hypertext transfer protocol: //www.boundless.com/microbiology/textbooks/boundless-microbiology-textbook/microbial-evolution-phylogeny-and-diversity-8/euryarchaeota-111/extremely-halophilic-archaea-575-7678/ ) .

  1. What does methyl i??-cyclodextrin bash to cell membranes? Based on this, explicate the consequence you would anticipate this compound to hold on Trypan Blue exclusion. Does your observation agree with this outlook? ( 4 Markss )

Cholesterol plays a really of import function in cell membranes as it is involved in keeping the permeableness and fluidness of membrane. Basically it gives the cell membrane excess support by maintaining the membrane fluid consistent. Methyl-?-cyclodextrin is a compound that is used to consume cells of cholesterin. Basically it removes cholesterin from membrane and disrupts membrane unity. Thus, I expect that Methyl-?-cyclodextrin would interrupt the membrane unity of the cells and damage the plasma membrane. As a consequence, the trypan blue can easy come in the cell and discoloration it. Furthermore, based on experimental informations from table 2.2, 78 % of cells with Methyl-?-cyclodextrin are stained will Trypan blue. Therefore, my observation agree with this outlook. hypertext transfer protocol: //www.sciencedirect.com/science/article/pii/S0005273608000837

  1. Predict what each of the undermentioned interventions will make to cell permeableness. Supply a brief ( 1-2 sentence ) account for each of your anticipations ( 1 Markss each ) :
  • Acetone: Acetone is an organic dissolver and organic dissolvers dissolve the lipoids of a membrane. Acetone is one of the common dissolvers for different organic molecules and has a greater ability to fade out lipoids than some other organic dissolvers like methyl alcohol. Acetone dissolves lipoids from cell membranes and makes them permeable to antibodies.
  • Methanol: Methanol is besides an organic dissolver which is used to fade out a membrane’s lipoid. However it is a weaker organic dissolver compared to acetone. It besides dissolves lipoids from cell membranes and makes them permeable to antibodies.
  • Saponin: Sapnion is a detergent which interacts with membrane cholesterin, selectively removes it and leaves holes inside the membrane.
  • Triton X-100: This is besides a detergent which interacts the membrane the same manner as Sapnion and it besides removes cholesterin. However there is besides a disadvantage of utilizing detergents like Triton X-100 because it is non-selective in nature and extracts proteins along the lipoids.

hypertext transfer protocol: //www.ncbi.nlm.nih.gov/pubmed/20012820

Bibliography

Fleet Digital M10T-MCS-MP Phase Contrast Tablet Microscope. ( n.d. ) . Retrieved May 18, 2015.

Retrieved from hypertext transfer protocol: //www.microscopeworld.com/p-2167-swift-digital-m10t-mcs-mp-phase-contrast-tablet-microscope.aspx

The Search engine I used is Google and the footings I used in hunt is phase contrast microscope with digital camera and the original mention is given below:

Fleet Digital M10T-MCS-MP Phase Contrast Tablet Microscope. ( n.d. ) . Retrieved May 18, 2015 Retrieved from hypertext transfer protocol: //www.microscopeworld.com/p-2167-swift-digital-m10t-mcs-mp-phase-contrast-tablet-microscope.aspx

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