chemical based plant growth promoter normally affect perticular organ
or function of plant, whereas a fermented product show effect on
the total plant system helping it to achieve better results as
compared to a chemical plant growth promoter and reduce the side
effects on the soil or the overall plant growth system. Plant growth
promoters could be harmful for human health if not applied in
adequate doses and convenient time on plants. In
present investigation leaves of various medicinal
plant e.g. Ricinus
Nicotiana tobacum (Tobacco)
taken in equal proportion and fermented for one month in cow urine
Filtered it and filtrate was taken as end product. Different
and 50 µl/ml)
of fermented product were applied to Mung (Vigna
seed germination. Growth
seeding were compared
showing that lower concentration increase seed germination and higher
concentration decrease seed germination than control (Without
treatment). We can conclude that this product can be use as growth
promoter at low concentration at germinatoin level.
recent years, use of continuous chemicals and intensive cultivation
have been reduced soil organic materials and micronutrients.It is
approved that continuance of these conditions lead to loss of
biological diversity, agro-ecosystem disorder and destruction of
soil structures.Plant active principles are chemical compounds
present in the entire plant or in specific parts of the plant that
confers them therapeutic activity or beneficial effects (Martins et
Hence use of this plant property to produce herbal growth promoter
is eco-friendly and cost effective.
In recent years, Thai farmers moslty used fermented plant extracts
(FPEs)as natural liquid fertilizers because it can easily produced
from agricultural products or agricultural waste. These FPEs can
promote plant growth and act as bio-control agents depending on the
type of plants being used (Kantachote and Charernjiratrakul, 2008).
Since FPEs are useful to reduce problems associated with the use of
chemical fertilizers and pesticides, they are now being widely
applied in organic agriculture, natural farming and IP farming.
there is very little scientific information to support the use of
FPEs. FPEs are product of lactic acid fermentation and most of the
available scientific information is concerned with the production and
use of them as beverages (Kantachote and Charernjiratrakul, 2008;
Prachyakij et al. 2008). Habitats and some physiological properties
of Lactic acid bacteria (LAB) and yeasts are confirmed that normally
found in fermented plant products (Oboh, 2006; Okada et al. 2006;
Olstorpe et al. 2008). We therefore thought of the possibility that
an FPE from leaves of medicinal plant might be useful as a potential
liquid fertilizer and could assist farmers because it would be easy
and cheap to prepare and make use of what is at present a common but
non utilized resource. Hence, the aims of this study were to
investigate effect of fermented pant extract on seed germinatin
Synthesis of plant
of various medicinal
plant e.g. Ricinus
Nicotiana tobacum (Tobacco)
taken in equal proportion (250gm)
and fermented for one month in cow urine (Gomutra).
Filtered it and filtrate was taken as end product.
can assess by a seed germination assay, it is one of the most common
techniques (Kapanen and Itavaara, 2001). The graded concentrations
(5,10,30 and 50 µl/ml)
plant extracts (FPEs) were added
aseptically to sterilized petriplateslined with Whatman no. 1 filter
paper. Surface sterilized seeds of Mung weregerminated (20 seed per
plate) in each concentration of PEFs. Similar experiment without
PEFwas conducted as control. Distilled water was used as a control
set for the testing of seed germination (Hoekstra et al. 2002;
Fuentes et al. 2004). After 10
days of treatment, seedlings were harvested and shoot and roots of
seedling were separated.Seedling growth in terms of root length,shoot
length, fresh weight and dry weightwere recorded and results were
compared to see effect of PEFs on seed germination and early seedling
pertaining to seed germination and
early seedling growth clearly indicate that PEFs at lower
concentration promoted seed germination and seedling growth, but at
higher concentration reduced seed germination and seedling growth.
Lower concentrations of PEFS (5,10
showed significant enhancement in shoot and root lengths, however
higher concentrations (50
of PEFsshowed decreased root length, shoot length and total seedlings
height. There was no major difference in root shoot ratio in all
treatments however an decreasing trend was seen from lower to higher
concentrations. Similar trend as in seedling height was seen in case
of fresh and dry weight of the seedlings after PEFs treatments
No.1 Effect of Fermented product on seedling growth in Mung
seedlings Height (cm)
Dry Wt. (mg)
Fruits and vegetables are a rich source of B (Bellaloui and Brown,
1998). Fermentation process has provided a relatively high amount of
plant nutrients particularly B, therefore after an appropriate
dilution the PFEs may be a suitable liquid fertilizer. It
has long been recognized that GAs play an important role in the
stimulation of seed germination (Chen et al. 2001), thereby the FPEs
may also induce seed germination. Other
plant nutrients particularly P, Mg, Mn and B may also be present at
appropriate concentrations to stimulate seed growth (Bellaloui and
Brown, 1998). The numbers of lactic acid bacteria (LAB) and yeasts
that were present during a wild forest noni (Morinda coreia Ham)
fermentation, the changes in its physicochemical properties and
levels of plant nutrients were investigated (Duangporn K et
.Higher concentratin decreases growth there may be increased
concentratin of citric acid.Results indicate that plant nutrients
present in the FPE were at an appropriate level for potential use as
a liquid fertilizer, particularly for the micronutrients such as B,
Mn and Zn.
this project, we can be concluded that fermented plant extract can
increase seed germination at lower concentration can use as herbal
growth promoter and at higher concentration decrease growth of
seedlings can used as herbicide. Based on these results, and because
of high chemical herbicide and growth regulator costs for farmers,
herbal growth promoter and herbicide can be replaced by them. In
addition to , they had not any environmental pollution and risks for
are thankful to Principal Dr. S B Patil, Hutatma Rajguru
Mahavidyalaya for his timely help and support.
Nacer and Brown, Patrick H. Cultivar differences in boron uptake and
distribution in celery (Apium graveolens), tomato (Lycopersicon
esculentum) and wheat (Triticum aestivum). Plant and Soil, January
1998, vol. 198, no. 2, p. 153-158.
Feng; Dahal, Peetambar and Bradford, Kent J. Two tomato expansion
genes show divergent expression and localization in embryos during
seed development and germination. Plant Physiology, November 2001,
vol. 127, no. 3, p. 928-936.
Kantachote,Kanjana Kowpong, Wilawan Charernjiratrakul Ashara
Pengnoo Microbial succession in a fermenting of wild forest noni
(Morinda coreia Ham) fruit plus m Ham) fruit plus molasses and its
role in producing a liquid fertilizer. Electronic Journal of
Biotechnology, June 2009,
Vol.12 No.3,p. 1-11.
Ana; Lloréns, Mercedes; Sáez, José; Aguilar, Maria Isabel;
Ortuño, Juan F. and Meseguer, Victor F. Phytotoxicity and heavy
metals speciation of stabilized sewage sludges. Journal of Hazardous
Materials, May 2004, vol. 108, no. 3, p. 161- 169.
N.J.; Bosker, T. and Lantinga, E.A. Effects of cattle dung from
farms with different feeding strategies on germination and initial
root growth of cress (Lepidium sativum L.). Agriculture Ecosystem
and Environment, December 2002, vol. 93, no. 1-3, p. 189-196.
ER, Castro DM, Castellani DC, Dias JE. Plantas medicinais. Viçosa,
MG: UFV; 2000.
Duangporn; Charernjiratrakul, Wilawan and Umsakul, Kamontam.
Antibacterial activities of fermented plant beverages collected in
southern Thailand. Journal of Biological Sciences, 2008, vol. 8, no.
8, p. 1280-1288.
A. and Itavaara, M. Ecotoxicity tests for compost applications.
Ecotoxicology and Environmental Safety, May 2001, vol. 49, no. 1, p.
Ganiyu. Nutrient enrichment of cassava peels using a mixed culture
of Saccharomyces cerevisiae and Lactobacillus spp. Solid media
fermentation techniques. Electronic Journal of Biotechnology,
January 2006, vol. 9, no. 1, p. 46-49.
Matilda; Lyberg, Karin; LIndberg Jan Erik; Schnurer, Johan and
Passoth, Volkmar Population diversity of yeasts and lactic acid
bacteria in pig feed fermented with whey, wet wheat distillers’
grains, or water at different temperatures. Applied and
Environmental Microbiology, March 2008, vol. 74, no. 6, p.
Hideki; Fukushi, Eri; Yamamon, Akira; Kawazoe, Naoki; Onodera,
Shuishi; Kawabata, Jun and Shiomi, Norio. Structural analysis of a
novel saccharide isolated from fermented beverage of plant extract.
Carbohydrate Research, May 2006, vol. 341, no. 7, p. 925-929.
Pakorn; Charernjiratrakul, Wilawan and Kantachote, Duangporn.
Improvement in the quality of a fermented seaweed beverage using an
antiyeast starter of Lactobacillus plantarum DW3 and partial
sterilization. World Journal of Microbiology and Biotechnology,
September 2008, vol. 24, no. 9, p. 1713- 1720.